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Establishment of hybridoma cell lines producing monoclonal antibodies against hepatitis B virus surface antigens (a, d, and r) and development of sensitive ELISA diagnostic test
Tang, XL; Zhang, XG; Xu, HM
2001-02-01
Source PublicationHYBRIDOMA
Volume20Issue:1Pages:47-52
AbstractA new treble-coated enzyme-linked immunoadsorbent assay (ELISA) kit of detecting Hepatitis B virus (HBV) surface antigen subtypes a, d and r (HBsAg-a, -d, -r) was developed by using four established hybridoma cell lines, of which two specifically secrete monoclonal antibodies (MAbs) against HBsAg-a (anti-HBsAg-a), one against -d (anti-HBsAg-d), and one against -r (anti-HBsAg-r). The approach of hybridoma cell lines' establishment were by fusing myeloma cells (SP2/0) with splenocytes from BALB/c mice immunized with a mixture of HBsAg-a, -d, -r, The ascitic MAb productivity of the four cell lines was at the titres of 1:10(6)-1:10(8). A treble-coated ELISA based HBV diagnostic kit was developed for detecting all of the three responding subtypes of HBsAgs. A 96-well ELISA microplate was coated with anti-HBSAg-a, -d, -r at a ratio of 3: 1: 0.5, with a horseradish peroxidase (HRP) conjugated anti-HBsAg-a as the labelled antibody. For clinical application, the new developed diagnostic kit detected HBsAgs of adr, adw, ayr, and ayw at a rate of lower than 0.25, 0.25, 0.5, and 0.5 ng/mL, respectively. Results indicated that this kit was more rapid and sensitive than that other current ELISA-based kits coated with a single MAb (e.g., anti-HBsAg-a).
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS Subject ExtendedBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
WOS KeywordTUMOR-CELLS ; EXPRESSION ; SPECIFICITY ; GENERATION ; CLONING ; MICE
Indexed BySCI
Language英语
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
WOS IDWOS:000168275400007
PublisherMARY ANN LIEBERT INC PUBL
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.igsnrr.ac.cn/handle/311030/68302
Collection中国科学院地理科学与资源研究所
Corresponding AuthorZhang, XG
Affiliation1.Beijing Biol & Immune Reagents Ctr, Beijing 100012, Peoples R China
2.Chinese Acad Sci, Commiss Integrated Survey Nat Resources, Beijing 100101, Peoples R China
3.Canadian Food Inspect Agcy, Ctr Anim & Plant Hlth, Charlottetown, PE C1A 5T1, Canada
Recommended Citation
GB/T 7714
Tang, XL,Zhang, XG,Xu, HM. Establishment of hybridoma cell lines producing monoclonal antibodies against hepatitis B virus surface antigens (a, d, and r) and development of sensitive ELISA diagnostic test[J]. HYBRIDOMA,2001,20(1):47-52.
APA Tang, XL,Zhang, XG,&Xu, HM.(2001).Establishment of hybridoma cell lines producing monoclonal antibodies against hepatitis B virus surface antigens (a, d, and r) and development of sensitive ELISA diagnostic test.HYBRIDOMA,20(1),47-52.
MLA Tang, XL,et al."Establishment of hybridoma cell lines producing monoclonal antibodies against hepatitis B virus surface antigens (a, d, and r) and development of sensitive ELISA diagnostic test".HYBRIDOMA 20.1(2001):47-52.
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