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Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties
Wang, Yonggang1,2; Li, Yuanli1; Li, Shaowei3; Li, QingYang4; Fan, Wenguang2; Kiatoukosin, Lormoua2; Chen, Jixiang1,5
2019-07-15
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume133Pages:172-183
Corresponding AuthorWang, Yonggang(412316788@163.com) ; Chen, Jixiang(chenjixiang@lut.cn)
AbstractThe yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways. An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima Fl. The production optimization, purification and characterization of the purified EPS from A. tenuissima Fl and the in-vitro antioxidant potentials was investigated. Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4. 7H20, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 C, inoculum size of 3%, 180 r/min for 5 d. The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 C by thermogravimetric analysis (TG/DSC). Molecular weight of the purified EPS was 3.246 x 104 g/mol, and EPS was composed of o-galacturonic acid, rhamnose, o-mannose, glucose, and o-galactose in ratio of 0.45:3.02:3.25:1.0:0.95. The EPS exhibited strong scavenging activity and may be a new source of natural antioxidant. (C) 2019 Elsevier B.V. All rights reserved.
KeywordEndophytic fungus exopolysaccharide Production optimization and purification Structure and bioactivities
DOI10.1016/j.ijbiomac.2019.03.246
WOS KeywordSTRUCTURAL-CHARACTERIZATION ; EXOPOLYSACCHARIDE PRODUCTION ; OPTIMIZATION ; CULTURE ; ANTITUMOR ; EXTRACTION ; PULLULAN
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31760028] ; National Natural Science Foundation of China[31460032] ; Youth Talent Support Program of Lanzhou University of Technology[2018]
Funding OrganizationNational Natural Science Foundation of China ; Youth Talent Support Program of Lanzhou University of Technology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000472685700021
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.igsnrr.ac.cn/handle/311030/58405
Collection中国科学院地理科学与资源研究所
Corresponding AuthorWang, Yonggang; Chen, Jixiang
Affiliation1.Lanzhou Univ Technol, Sch Energy & Power Engn, Lanzhou 730050, Gansu, Peoples R China
2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China
3.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China
4.Cent S Univ, Xiangya Sch Med, Changsha 410000, Hunan, Peoples R China
5.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Gansu, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yonggang,Li, Yuanli,Li, Shaowei,et al. Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,133:172-183.
APA Wang, Yonggang.,Li, Yuanli.,Li, Shaowei.,Li, QingYang.,Fan, Wenguang.,...&Chen, Jixiang.(2019).Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,133,172-183.
MLA Wang, Yonggang,et al."Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 133(2019):172-183.
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