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Recent advances in molecular techniques for the identification of phytopathogenic fungi - a mini review
Aslam, Sidra1; Tahir, Aisha2; Aslam, Muhammad Farhan3; Alam, Muhammad Waqar4; Shedayi, Arshad Ali5; Sadia, Sehrish1
2017-11-13
Source PublicationJOURNAL OF PLANT INTERACTIONS
ISSN1742-9145
Volume12Issue:1Pages:493-504
Corresponding AuthorAslam, Sidra(sidra.aslam29@yahoo.com)
AbstractAt present, 1.5 million species of fungi are estimated. Among these less than 5% have been described. Many fungal species cause disease in plants. These diseases cause major economic and production losses in the agricultural industry worldwide. Monitoring plant health and detecting the pathogen early are essential to reduce the disease spread, and facilitate effective management practices. DNA-based methods now provide essential tools for accurate plant disease diagnosis. Recently, effective amplification platforms, probe development, various quantitative PCR, DNA barcoding and RNA-Seq-based next-generation sequencing have revolutionized the research in fungal detection field, and differentiation area. Although the molecular diagnostics techniques have grown extensively over the last couple of decades but still there is a long way to go in the development and application of molecular diagnostics to assist the plant disease diagnosticians. Finally, molecular diagnostic techniques used in plant disease diagnostic clinics need to be robust, reliable, inexpensive and easy to be used that they can compete with, and complement traditional techniques. Challenge now remains residue with the researchers to develop the practical techniques used for diagnostic setting. Examples of the recent advancement in the molecular techniques for diagnosing the fungi causing plant disease are discussed in the review. Abbreviations: PCR: polymerase chain reaction; LAMP: loop-mediated isothermal amplification; RCA: rolling circle amplification; NASBA: nucleic acid sequence-based amplification; ITS: internal transcribed spacer; RT: reverse transcriptase; FEB: Fusarium ear blight; qPCR: real-time PCR; SNPs: single nucleotide polymorphism; HRCA: hyper-branched RCA; FIP: forward inner primer; BIP: backward inner primer; COX I: cytochrome c oxidase I; MBs: molecular beacons.
KeywordMolecular techniques fungal identification DNA barcoding molecular diagnostics PCR plant pathology
DOI10.1080/17429145.2017.1397205
WOS KeywordMEDIATED ISOTHERMAL AMPLIFICATION ; IN-SITU HYBRIDIZATION ; ROLLING-CIRCLE AMPLIFICATION ; POLYMERASE-CHAIN-REACTION ; INTERNAL TRANSCRIBED SPACER ; MAGNAPORTHE-GRISEA ; DIAGNOSTIC METHODS ; TISSUE SECTION ; DNA MICROARRAY ; RNA-SEQ
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31671321] ; National Natural Science Foundation of China[31371283]
Funding OrganizationNational Natural Science Foundation of China
WOS Research AreaPlant Sciences ; Environmental Sciences & Ecology
WOS SubjectPlant Sciences ; Ecology
WOS IDWOS:000425128300001
PublisherTAYLOR & FRANCIS LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.igsnrr.ac.cn/handle/311030/57039
Collection中国科学院地理科学与资源研究所
Corresponding AuthorAslam, Sidra
Affiliation1.Beijing Normal Univ, Coll Life Sci, MOE Key Lab Biodivers Sci & Ecol Engn, Beijing, Peoples R China
2.Univ Agr Faisalabad, Dept Biochem, Mol Biochem Lab, Faisalabad, Pakistan
3.Univ Agr Faisalabad, Natl Inst Biotechnol & Genet Engn, Hlth Biotechnol Div, Faisalabad, Pakistan
4.Univ Agr Faisalabad, Dept Plant Pathol, Faisalabad, Pakistan
5.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Aslam, Sidra,Tahir, Aisha,Aslam, Muhammad Farhan,et al. Recent advances in molecular techniques for the identification of phytopathogenic fungi - a mini review[J]. JOURNAL OF PLANT INTERACTIONS,2017,12(1):493-504.
APA Aslam, Sidra,Tahir, Aisha,Aslam, Muhammad Farhan,Alam, Muhammad Waqar,Shedayi, Arshad Ali,&Sadia, Sehrish.(2017).Recent advances in molecular techniques for the identification of phytopathogenic fungi - a mini review.JOURNAL OF PLANT INTERACTIONS,12(1),493-504.
MLA Aslam, Sidra,et al."Recent advances in molecular techniques for the identification of phytopathogenic fungi - a mini review".JOURNAL OF PLANT INTERACTIONS 12.1(2017):493-504.
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