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Rhizobia inhabiting nodules and rhizosphere soils of alfalfa: A strong selection of facultative microsymbionts
Wang, Xiao Lin1; Cui, Wen Jing1; Feng, Xue Ying1; Zhong, Zhi Ming2; Li, Ying1; Chen, Wen Xin1; Chen, Wen Feng1; Shao, Xiao Ming3; Tian, Chang Fu1
2018
Source PublicationSOIL BIOLOGY & BIOCHEMISTRY
ISSN0038-0717
Volume116Pages:340-350
Corresponding AuthorShao, Xiao Ming(shaoxm@cau.edu.cn) ; Tian, Chang Fu(cftian@cau.edu.cn)
AbstractAlfalfa (Medicago sativa L.) is an important leguminous forage crop, which is hypothesized to select compatible rhizobial partners from rhizosphere to form nitrogen-fixing nodules. Here we investigated the diversity of rhizobia from both nodules and rhizosphere of alfalfa from farmlands and natural ecosystems along the Yarlung Zangbo River valley in the Tibetan Plateau. 581 nodule isolates were characterized using BOX-A1R PCR fingerprinting, sequences of rpoB and nodulation gene nodC. They belong to four rpoB haplotypes of Sinorhizobium meliloti (tpoB-I, 473 isolates; tpoB-II, 95; rpoB-III, 1; rpoB-IV, 10), Sinorhizobium medicae (1 isolate) and Rhizobium sp. (1 isolate). Re-inoculation experiments of the 32 representative strains suggested a great variation in their symbiotic performance on alfalfa. High-throughput sequencing of rpoB for 34 rhizosphere samples uncovered that the predominant rpoB-I in nodules also dominated among fourteen rpoB haplotypes of S. meliloti in rhizosphere soils. In addition to S. meliloti, more than 40 rhizobial species documented as microsymbionts of other legumes were also identified. Although a very low level of nucleotide diversity for S. meliloti was found among sampling sites, multivariate statistical analysis demonstrated a significant differentiation of total rhizobial community between farmlands and natural ecosystems. Moreover, edaphic conditions, especially pH and nitrogen content, were revealed as important deterministic factors shaping the observed beta-diversity of rhizobial community. Taken together, these results demonstrated that the rhizospheric pool of rhizobial germplasms was significantly affected by land tillage compared to that of natural ecosystems, while a predominant population of S. meliloti was selected by alfalfa.
KeywordAlfalfa Nodule Rhizobia Rhizosphere rpoB
DOI10.1016/j.soilbio.2017.10.033
WOS KeywordSINORHIZOBIUM-MELILOTI ; GENETIC DIVERSITY ; MEDICAGO-TRUNCATULA ; SOYBEAN RHIZOSPHERE ; NITROGEN-FIXATION ; FIELD CONDITIONS ; TUNISIAN SOILS ; SEQUENCE DATA ; SP. NOV. ; LEGUMES
Indexed BySCI
Language英语
Funding ProjectProgram for New Century Excellent Talents in University[NCET-13-0561] ; National Basic Research Program of China (973 program)[2015CB158300] ; Chinese Universities Scientific Fund[2017SY003] ; Chinese Universities Scientific Fund[2017TC022] ; Key Research and Transferability Program in the Tibetan Autonomous Region[ZH20170001] ; State Key Laboratory of Agrobiotechnology ; Ministry of Science and Technology[2013BAD30B02-4]
Funding OrganizationProgram for New Century Excellent Talents in University ; National Basic Research Program of China (973 program) ; Chinese Universities Scientific Fund ; Key Research and Transferability Program in the Tibetan Autonomous Region ; State Key Laboratory of Agrobiotechnology ; Ministry of Science and Technology
WOS Research AreaAgriculture
WOS SubjectSoil Science
WOS IDWOS:000419417900037
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.igsnrr.ac.cn/handle/311030/56897
Collection中国科学院地理科学与资源研究所
Corresponding AuthorShao, Xiao Ming; Tian, Chang Fu
Affiliation1.China Agr Univ, Coll Biol Sci, Rhizobium Res Ctr, MOA Key Lab Soil Microbiol,State Key Lab Agrobiot, Beijing 100193, Peoples R China
2.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China
3.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xiao Lin,Cui, Wen Jing,Feng, Xue Ying,et al. Rhizobia inhabiting nodules and rhizosphere soils of alfalfa: A strong selection of facultative microsymbionts[J]. SOIL BIOLOGY & BIOCHEMISTRY,2018,116:340-350.
APA Wang, Xiao Lin.,Cui, Wen Jing.,Feng, Xue Ying.,Zhong, Zhi Ming.,Li, Ying.,...&Tian, Chang Fu.(2018).Rhizobia inhabiting nodules and rhizosphere soils of alfalfa: A strong selection of facultative microsymbionts.SOIL BIOLOGY & BIOCHEMISTRY,116,340-350.
MLA Wang, Xiao Lin,et al."Rhizobia inhabiting nodules and rhizosphere soils of alfalfa: A strong selection of facultative microsymbionts".SOIL BIOLOGY & BIOCHEMISTRY 116(2018):340-350.
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