IGSNRR OpenIR
High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress
Yin, Lina1,2,3; Mano, Jun'ichi4,5; Tanaka, Kiyoshi3; Wang, Shiwen1,2; Zhang, Meijuan1; Deng, Xiping1,2; Zhang, Suiqi1,2
2017-10-01
Source PublicationPHYSIOLOGIA PLANTARUM
ISSN0031-9317
Volume161Issue:2Pages:211-223
Corresponding AuthorYin, Lina(linayin@nwsuaf.edu.cn)
AbstractLipid peroxide-derived reactive carbonyl species (RCS), generated downstream of reactive oxygen species (ROS), are critical damage-inducing species in plant aluminum (Al) toxicity. In mammals, RCS are scavenged primarily by glutathione (reduced form of glutathione, GSH), but in plant Al stress, contribution of GSH to RCS detoxification has not been evaluated. In this study, Arabidopsis plants overexpressing the gene AtGR1 (accession code At3g24170), encoding glutathione reductase (GR), were generated, and their performance under Al stress was examined. These transgenic plants (GR-OE plants) showed higher GSH levels and GSH/GSSG (oxidized form of GSH) ratio, and an improved Al tolerance as they suffered less inhibition of root growth than wild-type under Al stress. Exogenous application of 4-hydroxy-2-nonenal, an RCS responsible for Al toxicity in roots, markedly inhibited root growth in wild-type plants. GR-OE plants suffered significantly smaller inhibition, indicating that the enhanced GSH level increased the capacity of RCS detoxification. The generation of H2O2 due to Al stress in GR-OE plants was lower by 26% than in wild-type. Levels of various RCS, such as malondialdehyde, butyraldehyde, phenylacetaldehyde, (E)-2-heptenal and n-octanal, were suppressed by more than 50%. These results indicate that high levels of GSH and GSH/GSSG ratio by GR overexpression contributed to the suppression of not only ROS, but also RCS. Thus, the maintenance of GSH level by overexpressing GR reinforces dual detoxification functions in plants and is an efficient approach to enhance Al tolerance.
DOI10.1111/ppl.12583
WOS KeywordWHEAT TRITICUM-AESTIVUM ; OXIDATIVE STRESS ; SUPEROXIDE-DISMUTASE ; PHOTOOXIDATIVE STRESS ; SENSITIVE METHOD ; TOLERANCE ; PLANTS ; RESISTANCE ; CHLOROPLASTS ; TOXICITY
Indexed BySCI
Language英语
Funding ProjectNational Natural Sciences Foundation of China[31200206] ; Northwest AF University ; State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau[A314021403-C5] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2015389] ; West Light Foundation of the Chinese Academy of Sciences
Funding OrganizationNational Natural Sciences Foundation of China ; Northwest AF University ; State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; West Light Foundation of the Chinese Academy of Sciences
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000417402000003
PublisherWILEY
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.igsnrr.ac.cn/handle/311030/56795
Collection中国科学院地理科学与资源研究所
Corresponding AuthorYin, Lina
Affiliation1.Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Inst Soil & Water Conservat, Yangling 712100, Peoples R China
2.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Peoples R China
3.Tottori Univ, Fac Agr, Tottori 6808533, Japan
4.Yamaguchi Univ, Sci Res Ctr, Yamaguchi 7538515, Japan
5.Yamaguchi Univ, Grad Sch Agr, Yamaguchi 7538515, Japan
Recommended Citation
GB/T 7714
Yin, Lina,Mano, Jun'ichi,Tanaka, Kiyoshi,et al. High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress[J]. PHYSIOLOGIA PLANTARUM,2017,161(2):211-223.
APA Yin, Lina.,Mano, Jun'ichi.,Tanaka, Kiyoshi.,Wang, Shiwen.,Zhang, Meijuan.,...&Zhang, Suiqi.(2017).High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress.PHYSIOLOGIA PLANTARUM,161(2),211-223.
MLA Yin, Lina,et al."High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress".PHYSIOLOGIA PLANTARUM 161.2(2017):211-223.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yin, Lina]'s Articles
[Mano, Jun'ichi]'s Articles
[Tanaka, Kiyoshi]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yin, Lina]'s Articles
[Mano, Jun'ichi]'s Articles
[Tanaka, Kiyoshi]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yin, Lina]'s Articles
[Mano, Jun'ichi]'s Articles
[Tanaka, Kiyoshi]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.