IGSNRR OpenIR
MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN
Fayyaz, Amna1; Tufail, Muhammad Rizwan1; Sahi, Shahbaz Talib1; Masood, Atifa2; Tazeen, Syeda Khola2; Naseer, Ignaa3; Farooq, Taimoor Hassan4; Amrao, Luqman1
2019
Source PublicationFRESENIUS ENVIRONMENTAL BULLETIN
ISSN1018-4619
Volume28Issue:3Pages:1770-1774
Corresponding AuthorMasood, Atifa(dratifabot@yahoo.com)
AbstractAmong many other plant diseases Chili Leaf Curl Disease (ChLCD) caused by Begomovirus complex is a crucial threat for chili worldwide. Chili Leaf Curl Virus (ChLCV) is monopartites DNA Begomovirus having DNA-A which comprises of 2754 nucleotides having six ORFs and betasetallite of size 1.3kb. Genomic DNA of infected chili leaf samples was extracted by CTAB method, collected from different field areas of Faisalabad. Circular molecules from genomic DNA were amplified by using Rolling Circle Amplification (RCA). Three samples showed successful RCA which further used in PCR amplification. For amplification of full length Begomovirus from RCA product universal primers were used. Betasatellite component associated with ChLCD was amplified with the help of PCR by using full-length universal primers 0301 and 002). RFLP analysis showed that single type of Begomovirus and diverse type of betasatellite molecules observed in infected chili samples.
KeywordChili Leaf Curl Disease Begomoviruses Chili Leaf Curl Virus Betasatellite RFLP
WOS Keyword1ST REPORT ; GENETIC DIVERSITY ; DNA-BETA ; BEGOMOVIRUS ; DISEASE ; TOMATO ; PEPPER ; VECTOR
Indexed BySCI
Language英语
Funding ProjectHigher Education Commission, Pakistan
Funding OrganizationHigher Education Commission, Pakistan
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000463297500015
PublisherPARLAR SCIENTIFIC PUBLICATIONS (P S P)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.igsnrr.ac.cn/handle/311030/48202
Collection中国科学院地理科学与资源研究所
Corresponding AuthorMasood, Atifa
Affiliation1.Univ Agr Faisalabad, Dept Plant Pathol, Faisalabad, Pakistan
2.Univ Lahore, Dept Bot, Sargodha, Punjab, Pakistan
3.Univ Chinese Acad Sci, Inst Geog Sci & Nat Resources, Key Lab Ecosyst Res Network Observat & Modeling, Beijing, Peoples R China
4.Fujian Agr & Forestry Univ, Forestry & Environm Res Inst, Fuzhou, Fujian, Peoples R China
Recommended Citation
GB/T 7714
Fayyaz, Amna,Tufail, Muhammad Rizwan,Sahi, Shahbaz Talib,et al. MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2019,28(3):1770-1774.
APA Fayyaz, Amna.,Tufail, Muhammad Rizwan.,Sahi, Shahbaz Talib.,Masood, Atifa.,Tazeen, Syeda Khola.,...&Amrao, Luqman.(2019).MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN.FRESENIUS ENVIRONMENTAL BULLETIN,28(3),1770-1774.
MLA Fayyaz, Amna,et al."MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN".FRESENIUS ENVIRONMENTAL BULLETIN 28.3(2019):1770-1774.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Fayyaz, Amna]'s Articles
[Tufail, Muhammad Rizwan]'s Articles
[Sahi, Shahbaz Talib]'s Articles
Baidu academic
Similar articles in Baidu academic
[Fayyaz, Amna]'s Articles
[Tufail, Muhammad Rizwan]'s Articles
[Sahi, Shahbaz Talib]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Fayyaz, Amna]'s Articles
[Tufail, Muhammad Rizwan]'s Articles
[Sahi, Shahbaz Talib]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.