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Rare microbial taxa rather than phoD gene abundance determine hotspots of alkaline phosphomonoesterase activity in the karst rhizosphere soil
Liu, Shuang1,2; Zhang, Xinyu1,2; Dungait, Jennifer A. J.3; Quine, Timothy A.3; Razavi, Bahar S.4
2020-11-14
Source PublicationBIOLOGY AND FERTILITY OF SOILS
ISSN0178-2762
Pages12
Corresponding AuthorZhang, Xinyu(zhangxy@igsnrr.ac.cn)
AbstractThe spatial distribution of alkaline phosphomonoesterase (ALP) activity in the rhizosphere and bulk soil of three plants (Zea mays, Medicago sativa, and Cyperus rotundus) grown in a karst soil (pH 8.27) was visualized using in situ soil zymography. According to the zymogram images, we identified and precisely collected soil samples from hotspots and non-hotspots of ALP activity, and then analyzed the phoD genes that encoded alkaline phosphomonoesterase and assessed the microbial community. The results showed that (1) the phoD abundance in the plant types varied and was highest in the alfalfa and lowest in the maize; (2) Proteobacteria dominated the phoD-harboring microbes in the rhizosphere and Actinobacteria dominated the phoD-harboring microbes in the bulk soil, and (3) the ALP activity was positively correlated with the relative abundance of the diazotrophic Azotobacter, but negatively correlated with the phoD gene abundance, microbial community richness, and diversity. By coupling zymography and microbial molecular approaches, we identified hotspots of enzymatic and microbial activity in rhizosphere soil and evaluated the relative contributions from potential active microorganisms. We found that the function of specific phoD-harboring microorganisms in these hotspots differed depending on the plant in the soil, which had implications for phosphorus (P) management in P-limited karst soils. The results also suggested that free-living N-2-fixing bacteria (Azotobacter) might promote ALP activity, thereby emphasizing vital linkages between, and coupling of, the soil nutrient cycles.
KeywordphoD gene Phosphate mineralizing Microbial hotspots Rhizosphere Soil zymography
DOI10.1007/s00374-020-01522-4
WOS KeywordENZYME-ACTIVITY ; PHOSPHORUS FERTILIZATION ; SPATIAL-DISTRIBUTION ; RESOURCE LIMITATION ; PLANT-GROWTH ; MICROORGANISMS ; COMMUNITIES ; MECHANISMS ; ZYMOGRAPHY ; DIVERSITY
Indexed BySCI
Language英语
Funding ProjectScience Centre project of National Natural Science Foundation of China[31988102] ; National Natural Science Foundation of China[41830860] ; National Natural Science Foundation of China[41877091] ; National Natural Science Foundation of China[41571130043] ; National Environmental Research Council of the UK[NE/N007603/1]
Funding OrganizationScience Centre project of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Environmental Research Council of the UK
WOS Research AreaAgriculture
WOS SubjectSoil Science
WOS IDWOS:000589505300001
PublisherSPRINGER
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.igsnrr.ac.cn/handle/311030/156558
Collection中国科学院地理科学与资源研究所
Corresponding AuthorZhang, Xinyu
Affiliation1.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100190, Peoples R China
3.Univ Exeter, Coll Life & Environm Sci, Geog, Rennes Dr, Exeter EX4 4RJ, Devon, England
4.Univ Kiel, Inst Phytopathol, Dept Soil & Plant Microbiome, Kiel, Germany
Recommended Citation
GB/T 7714
Liu, Shuang,Zhang, Xinyu,Dungait, Jennifer A. J.,et al. Rare microbial taxa rather than phoD gene abundance determine hotspots of alkaline phosphomonoesterase activity in the karst rhizosphere soil[J]. BIOLOGY AND FERTILITY OF SOILS,2020:12.
APA Liu, Shuang,Zhang, Xinyu,Dungait, Jennifer A. J.,Quine, Timothy A.,&Razavi, Bahar S..(2020).Rare microbial taxa rather than phoD gene abundance determine hotspots of alkaline phosphomonoesterase activity in the karst rhizosphere soil.BIOLOGY AND FERTILITY OF SOILS,12.
MLA Liu, Shuang,et al."Rare microbial taxa rather than phoD gene abundance determine hotspots of alkaline phosphomonoesterase activity in the karst rhizosphere soil".BIOLOGY AND FERTILITY OF SOILS (2020):12.
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