Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale
Wang, Ziquan1,2; Tian, Haixia1; Lei, Mei3; Megharaj, Mallavarapu4; Tan, Xiangping1,5; Wang, Fang2,6; Jia, Hanzhong1; He, Wenxiang1
Corresponding AuthorJia, Hanzhong(jiahz@nwafu.edu.cn) ; He, Wenxiang(wenxiang.he@nwafu.edu.cn)
AbstractInformation on the kinetic characteristics of soil enzymes under long-term arsenic (As) pollution in field soils is scarce. We investigated Michaelis-Menten kinetic properties of four soil enzymes including beta-glucosidase (BG), acid phosphatase (ACP), alkaline phosphatase (ALP), and dehydrogenase (DHA) in field soils contaminated by As resulting from long-term realgar mining activity. The kinetic parameters, namely the maximum reaction velocity (V-max), enzyme-substrate affinity (K-m) and catalytic efficiency (V-max/K-m) were calculated. Results revealed that the enzyme kinetic characteristics varied in soils and were significantly influenced by total nitrogen (N) and total As, which explained 31.8% and 30.7% of the variance in enzyme kinetics respectively. Enzyme pools (V-max) and catalytic efficiency (V-max/K-m) of BG, ACP and DHA decreased with elevated As pollution, while the enzyme affinity for substrate (K-m) was less affected. Redundancy analysis and stepwise regression suggested that the adverse influence of As on enzyme kinetics may offset or weakened by soil total N and soil organic matter (SOM). Concentration-response fitting revealed that the specific kinetic parameters expressed as the absolute enzyme kinetic parameters multiplied by normalized soil total N and SOM were more relevant than the absolute ones to soil total As. The arsenic ecological dose values that cause 10% decrease (ED10) in the specific enzyme kinetics were 20-49 mg kg(-1), with a mean value of 35 mg kg(-1), indicating a practical range of threshold for As contamination at field level. This study concluded that soil enzymes exhibited functional adaptation to long-term As stress mainly through the reduction of enzyme pools (Vmax) or maintenance of enzyme-substrate affinity (K-m). Further, this study demonstrates that the specific enzyme kinetics are the better indicators of As ecotoxicity at field-scale compared with the absolute enzyme parameters.
KeywordField soil pollution Catalytic efficiency Risk assessment Bioindicator Ecological dose
Indexed BySCI
Funding ProjectNational Natural Science Foundation of China[41571245] ; National Natural Science Foundation of China[41603116] ; National Natural Science Foundation of China[31600384] ; Basic Scientific Research Foundation of Northwest A F University[ZD2013012] ; China Postdoctoral Science Foundation[2016M592549]
Funding OrganizationNational Natural Science Foundation of China ; Basic Scientific Research Foundation of Northwest A F University ; China Postdoctoral Science Foundation
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000514011400011
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorJia, Hanzhong; He, Wenxiang
Affiliation1.Northwest A&F Univ, Coll Nat Resources & Environm, Key Lab Plant Nutr & Agroenvironm Northwest China, Minist Agr, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Peoples R China
3.Chinese Acad Sci, Ctr Environm Remediat, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China
4.Univ Newcastle, Fac Sci, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
5.Chinese Acad Sci, Key Lab Vegetat Restorat & Management Degraded Ec, South China Bot Garden, 723 Xingke Rd, Guangzhou 510650, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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GB/T 7714
Wang, Ziquan,Tian, Haixia,Lei, Mei,et al. Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2020,191:8.
APA Wang, Ziquan.,Tian, Haixia.,Lei, Mei.,Megharaj, Mallavarapu.,Tan, Xiangping.,...&He, Wenxiang.(2020).Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,191,8.
MLA Wang, Ziquan,et al."Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 191(2020):8.
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