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Responses of C-, N- and P-acquiring hydrolases to P and N fertilizers in a subtropical Chinese fir plantation depend on soil depth
Zhang, Chuang1,5; Zhang, Xinyu1,6; Kuzyakov, Yakov2,5,7,8; Wang, Huimin1,6; Fu, Xiaoli1,6; Yang, Yang1,6; Chen, Fusheng3; Dungait, Jennifer A. J.4; Green, Sophie M.4; Fang, Xiangmin3,9
2020-06-01
Source PublicationAPPLIED SOIL ECOLOGY
ISSN0929-1393
Volume150Pages:7
Corresponding AuthorZhang, Xinyu(zhangxy@igsnrr.ac.cn)
AbstractLarge amounts of carbon (C), nitrogen (N) and phosphorus (P) in soil are stored in forms that are not bioavailable unless they are modified by hydrolytic enzymes. The activity of hydrolases is affected directly and indirectly by fertilizers treated, but the effects depend on soil depth. We collected soil core (0-80 cm depth) from fertilized and unfertilized (control) plots in a subtropical Chinese fir plantation. The soil with fertilizers had been treated for five years with either N (50 kg N ha(-1) yr(-1)), P (50 kg P ha(-1) yr(-1)) or N and P together. The kinetics of three hydrolases (beta-1,4-glucosidase (beta Gluc), beta-1,4-N-acetylglucosaminidase (NAG) and acid phosphatase (Phos)) were measured, and their potential activities (V-max), half-saturation constants (K-m), and enzyme efficiencies (V-max/K-m) were calculated for 0-10 cm, 10-20 cm, 20-40 cm, 40-60 cm, and 60-80 cm soil depth increments. The effect of depth on enzyme kinetics was greater than fertilizers treated. Smaller soil organic carbon (SOC) contents were related to the stable efficiencies of NAG and Phos from 0 to 60 cm in the control plots. In general, decreases in SOC content with depth triggered the production of enzymes with low K-m (i.e. high substrate affinity). The minimum and maximum decreases in the K-m of beta Gluc, NAG and Phos were 0%, 22% and 64% in the top 20 cm and 30%, 54% and 79% at 80 cm depth, respectively, compared to 0-10 cm soil depth. Nitrogen fertilizer downregulated NAG production without changing K-m with soil depth, leading to decreased V-max and V-max/K-m compared to the control. Responses of Phos to P and NP fertilizers depended on soil organic matter (SOM) and available P contents. Relative to the control, the Phos V-max decreased in the top 10 cm, increased between 10 and 40 cm, and lack of difference between 40 and 80 cm in the P- and NP-treated soils. The V-max and V-max/K-m of beta Gluc increased by P fertilizers between 60 and 80 cm compared to the control, indicating microbial mobilization of P from SOM to meet energy and C requirements in nutrient-poor subsoil. We conclude that limitations in microbial activity caused by decreases in SOM with depth with N and P fertilizers Chinese fir plantations are offset by the production of more efficient enzymes. However, longer term management to build SOM in tree plantations is required to prevent further soil degradation and loss of productivity.
KeywordNitrogen and phosphorus fertilizers Soil organic matter Soil depth Hydrolase kinetic parameters Subtropical soil Chinese fir plantation
DOI10.1016/j.apsoil.2019.103465
WOS KeywordLAND-USE CHANGE ; EXTRACELLULAR ENZYME-ACTIVITIES ; MICROBIAL COMMUNITY STRUCTURE ; TERM NITROGEN DEPOSITION ; PHOSPHORUS LIMITATION ; CARBON DYNAMICS ; ORGANIC-CARBON ; BIOMASS ; KINETICS ; ADDITIONS
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41571251] ; National Natural Science Foundation of China[41830860] ; National Natural Science Foundation of China[41877091] ; National Natural Science Foundation of China[41671253] ; Government Program of Competitive Growth of Kazan Federal University ; RUDN University program 5-100
Funding OrganizationNational Natural Science Foundation of China ; Government Program of Competitive Growth of Kazan Federal University ; RUDN University program 5-100
WOS Research AreaAgriculture
WOS SubjectSoil Science
WOS IDWOS:000512886800019
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.igsnrr.ac.cn/handle/311030/132409
Collection中国科学院地理科学与资源研究所
Corresponding AuthorZhang, Xinyu
Affiliation1.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing 100101, Peoples R China
2.Huazhong Agr Univ, Coll Resources & Environm, Wuhan 430070, Peoples R China
3.Jiangxi Agr Univ, Coll Forestry, Nanchang 330045, Jiangxi, Peoples R China
4.Univ Exeter, Coll Life & Environm Sci, Geog, Rennes Dr, Exeter EX4 4RJ, Devon, England
5.Chinese Acad Sci, Ctr Agr Resources Res, Inst Genet & Dev Biol, Key Lab Agr Water Resources, 286 Huaizhong Rd, Shijiazhuang 050021, Hebei, Peoples R China
6.Univ Chinese Acad Sci Beijing, Coll Resources & Environm, Beijing 100190, Peoples R China
7.RUDN Univ, Agrotechnol Inst, Moscow 117198, Russia
8.Kazan Fed Univ, Inst Environm Sci, Kazan 420049, Russia
9.Univ La Frontera, Dept Ciencias Quim & Recursos Nat, Temuco, Chile
Recommended Citation
GB/T 7714
Zhang, Chuang,Zhang, Xinyu,Kuzyakov, Yakov,et al. Responses of C-, N- and P-acquiring hydrolases to P and N fertilizers in a subtropical Chinese fir plantation depend on soil depth[J]. APPLIED SOIL ECOLOGY,2020,150:7.
APA Zhang, Chuang.,Zhang, Xinyu.,Kuzyakov, Yakov.,Wang, Huimin.,Fu, Xiaoli.,...&Fang, Xiangmin.(2020).Responses of C-, N- and P-acquiring hydrolases to P and N fertilizers in a subtropical Chinese fir plantation depend on soil depth.APPLIED SOIL ECOLOGY,150,7.
MLA Zhang, Chuang,et al."Responses of C-, N- and P-acquiring hydrolases to P and N fertilizers in a subtropical Chinese fir plantation depend on soil depth".APPLIED SOIL ECOLOGY 150(2020):7.
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