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SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza
Li, Wenrui1,2; Xing, Bingcong1,2; Mao, Renjun3; Bai, Zhenqing3; Yang, Dongfeng4; Xu, Jiayi3; Liang, Zongsuo1,4
2020-02-01
Source PublicationINDUSTRIAL CROPS AND PRODUCTS
ISSN0926-6690
Volume144Pages:11
Corresponding AuthorLiang, Zongsuo(liangzs@ms.iswc.ac.cn)
AbstractSalvia miltiorrhiza Bunge is the origin plant of traditional Chinese medicine, Danshen. Demand of high-quality S. miltiorrhiza is increasing because of its excellent pharmaceutical value. Tanshinones and salvianolic acids (SAs) are the main active ingredients of S. miltiorrhiza. GA was reported to induce tanshinones and SAs accumulation, however, its mechanism was unclear. Here, we got a GA responsive protein, SmGRAS3. SmGRAS3 expressed highest in the periderm where tanshinones produced and accumulated. Results of SmGRAS3 over and antisense expression indicated that SmGRAS3 is a positive regulator of tanshinones biosynthesis in S. miltiorrhiza hairy roots. But overexpression of SmGRAS3 depressed SAs and GA biosynthesis as well as hairy roots growth, and application of GA could partially recover these depressions. Pathway enzyme genes expressions of tanshinones, SAs and GA biosynthesis were comprehensively changed in SmGRAS3 transgenic hairy roots. SmGRAS3 acted as a transcription factor that located in nucleus and has the transcription activation activity. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assay (EMSA) showed that SmGRAS3 directly bound to GARE-motif in promoter of KSL1 and activated KSL1 transcription. Our study suggested that the SmGRAS3 may be a good potential target for further metabolic engineering of bioactive components biosynthesis in S. miltiorrhiza.
KeywordSalvia mildorrhiza Tanshinones GRAS GA signaling Regulation
DOI10.1016/j.indcrop.2019.112004
WOS KeywordCHINESE MEDICINAL PREPARATIONS ; GRAS TRANSCRIPTION FACTOR ; HAIRY ROOT CULTURES ; PHENOLIC-ACIDS ; SYNTHASE GENE ; EXPRESSION ; ACCUMULATION ; SCARECROW ; PROTEINS ; INTERACTS
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81373908] ; Ministry of Science and Technology of the People's Republic of China through the Twelfth Five-Year National Science and Technology Pillar Program[2015BACO1B03]
Funding OrganizationNational Natural Science Foundation of China ; Ministry of Science and Technology of the People's Republic of China through the Twelfth Five-Year National Science and Technology Pillar Program
WOS Research AreaAgriculture
WOS SubjectAgricultural Engineering ; Agronomy
WOS IDWOS:000510521700010
PublisherELSEVIER
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.igsnrr.ac.cn/handle/311030/132223
Collection中国科学院地理科学与资源研究所
Corresponding AuthorLiang, Zongsuo
Affiliation1.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Shaanxi, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
4.Zhejiang Sci Tech Univ, Coll Life Sci & Med, Hangzhou 310018, Peoples R China
Recommended Citation
GB/T 7714
Li, Wenrui,Xing, Bingcong,Mao, Renjun,et al. SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza[J]. INDUSTRIAL CROPS AND PRODUCTS,2020,144:11.
APA Li, Wenrui.,Xing, Bingcong.,Mao, Renjun.,Bai, Zhenqing.,Yang, Dongfeng.,...&Liang, Zongsuo.(2020).SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza.INDUSTRIAL CROPS AND PRODUCTS,144,11.
MLA Li, Wenrui,et al."SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza".INDUSTRIAL CROPS AND PRODUCTS 144(2020):11.
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