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SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza
Li, Wenrui1,2; Bai, Zhenqing3; Pei, Tianlin3; Yang, Dongfeng4; Mao, Renjun3; Zhang, Bingxue1,2; Liu, Chuangfeng3; Liang, Zongsuo1,4
2019-10-30
Source PublicationFRONTIERS IN PLANT SCIENCE
ISSN1664-462X
Volume10Pages:13
Corresponding AuthorLiang, Zongsuo(liangzs@ms.iswc.ac.cn)
AbstractSalvia miltiorrhiza is one of the most widely used traditional Chinese medicinal plants because of its excellent performance in treating heart diseases. Tanshinones and phenolic acids are two important classes of effective metabolites, and their biosynthesis has attracted widespread interest. Here, we functionally characterized SmGRAS1 and SmGRAS2, two GRAS family transcription factors from S. miltiorrhiza. SmGRAS1/2 were highly expressed in the root periderm, where tanshinones mainly accumulated in S. miltiorrhiza. Overexpression of SmGRAS1/2 upregulated tanshinones accumulation and downregulated GA, phenolic acids contents, and root biomass. However, antisense expression of SmGRAS1/2 reduced the tanshinones accumulation and increased the GA, phenolic acids contents, and root biomass. The expression patterns of biosynthesis genes were consistent with the changes in compounds accumulation. GA treatment increased tanshinones, phenolic acids, and GA contents in the overexpression lines, and restored the root growth inhibited by overexpressing SmGRAS1/2. Subsequently, yeast one-hybrid, dual-luciferase, and electrophoretic mobility shift assays (EMSA) showed SmGRAS1 promoted tanshinones biosynthesis by directly binding to the GARE motif in the SmKSL1 promoter and activating its expression. Yeast two-hybrid assays showed SmGRAS1 interacted physically with SmGRAS2. Taken together, the results revealed that SmGRAS1/2 acted as repressors in root growth and phenolic acids biosynthesis but as positive regulators in tanshinones biosynthesis. Overall, our findings revealed the potential value of SmGRAS1/2 in genetically engineering changes in secondary metabolism.
KeywordSalvia miltiorrhiza SmGRAS1 2 GA tanshinines phenolic acids biosynthesis
DOI10.3389/fpls.2019.01367
WOS KeywordHAIRY ROOTS ; GIBBERELLIN ; GENE ; ACCUMULATION ; EXPRESSION ; MECHANISM ; PATHWAY ; FAMILY ; MODULE ; GROWTH
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81373908] ; Ministry of Science and Technology of the People's Republic of China through the Twelfth Five-Year National Science and Technology Pillar Program[2015BAC01B03]
Funding OrganizationNational Natural Science Foundation of China ; Ministry of Science and Technology of the People's Republic of China through the Twelfth Five-Year National Science and Technology Pillar Program
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000496167000001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.igsnrr.ac.cn/handle/311030/131845
Collection中国科学院地理科学与资源研究所
Corresponding AuthorLiang, Zongsuo
Affiliation1.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling, Shaanxi, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Northwest A&F Univ, Coll Life Sci, Yangling, Shaanxi, Peoples R China
4.Zhejiang Sci Tech Univ, Coll Life Sci & Med, Hangzhou, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Li, Wenrui,Bai, Zhenqing,Pei, Tianlin,et al. SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza[J]. FRONTIERS IN PLANT SCIENCE,2019,10:13.
APA Li, Wenrui.,Bai, Zhenqing.,Pei, Tianlin.,Yang, Dongfeng.,Mao, Renjun.,...&Liang, Zongsuo.(2019).SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza.FRONTIERS IN PLANT SCIENCE,10,13.
MLA Li, Wenrui,et al."SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza".FRONTIERS IN PLANT SCIENCE 10(2019):13.
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